Investigation of creatine’s modulatory effect on calcium quantitative alterations in hippocampus under the long-term psycho-emotional stress

Author: Temur Mantashashvili
Co-authors: Mantashashvili Temuri, Tsiklauri Aleksandre, Burjanadze George, Menabde Ketevan, Koshoridze Nana
Keywords: Creatine, psycho-emotional stress, Calcium, Brain, Nitric Oxide
Annotation:

Circadian rhythm disturbance is an important stress factor that can adversely affect the living system. Similar external factors affect both the metabolic processes and the quantitative change of various signaling agents, among which Ca2+ plays an important role. It is known that the quantitative changes of Ca2+ may have a cytotoxic effect, adversely affect the normal functioning of mitochondria and cause the activation of oxidative processes. Given the complexity of these variations, it is important to find and study biologically active compounds that perform certain protective functions, including nitrogenous creatine involved in the transport and storage of high-energy phosphates in the cell. The aim of the study was to study the quantitative changes of Ca2+ in the hippocampus of the white rat, to observe some oxidative processes and to determine the effect of creatine on these parameters. It has been shown that in the hippocampus of the stressed group (G2), the concentration of Ca2+ is significantly increased compared to the control group (G1), and creatine administration (G3) is making the parameter closer to the control value. It should also be noted that under the circadian rhythm violation, there is a significant increase in active radicals such as nitric oxide (NO) and hydrogen peroxide (H2O2), with creatine supply also having a positive effect. Along with the above, cell viability was studied, which was significantly reduced in G2 animals compared to G1, and in the G3 group there was a significant improvement. Oxidative stress induced by long-term disturbances in natural circadian rhythm is accompanied and likely provoked by an increase in Ca2+ levels. Therefore, creatine impact on energy mediating pathways has a positive effect on stabilization of antioxidant, as well as different metabolic systems, thus protecting the hippocampal cells from further stress impact.